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Cytogenetic Service

Scientific Coordinator

Jesús María Hernández Rivas

+34 923 294 812

jmhr@usal.es

Molecular Cytogenetic Service

Centro de Investigación del Cáncer (CSIC-USAL).

Campus Universitario Miguel de Unamuno s/n E-37007 Salamanca (Spain)

Personnel

No information is available

The Molecular Cytogenetic Service (MCU) is a facility devoted to the karyotypic analysis, fluorescence «in situ» hybridization, comparative genomic hybridization, microarrays and next generation sequencing of cancer patients. More than 100 hospitals in Spain, and occasionally others from the EU, have used the MCU services.

The Unit collaborates with the most relevant groups in the treatment of the hematological malignancies such as Pethema, GEL-TAMO, GEM or GETH providing technical support and characterization of the genetic abnormalities in the patients included in clinical trials. In addition, the MCU is involved in several international projects related to expression microarrays (MILE), genomic microarrays (EuGESMA), next generation sequencing (IRON, ELAN, NGS-PTL), and the European project HARMONY, which through the use of Big Data will help improve the care of patients with hematologic malignancies.

In this Facility we provide complete services for:

  • Bone Marrow Cytogenetic: leukemia, lymphoma and myeloma.

  • Peripheral blood cytogenetic: leukemia, lymphoma and myeloma.

  • Lymph node and spleen cytogenetics: lymphoma.

  • Solid tumour cytogenetics.

  • Centromeric «in situ» hybridization: FISH performed with centromeric probes to analyse numerical abnormalities.

  • Painting «in situ» hybridization: FISH performed with libraries of DNA to analyse structural abnormalities.

  • Loci specific «in situ» hybridizations: FISH performed with probes to analyse either losses or gains of genetic material or fusion genes.

  • Comparative Genomic Hybridization: Test to analyse global gains and losses of genetic material in tumour cells.

  • Genomic microarrays.

  • Pharmacogenetic microarrays.

  • Next generation sequencing to analyses genetic alterations by flexible and scalable methodology for target regions, genes, individual exons or hot spots. Available disease-focused panels (custom and commercial), fusion gene panels and RNA seq.

  • High-Depth next-generation sequencing for somatic variant detection.

  • Germline variant detection by next-generation sequencing in key genes involved in severe congenital coagulationbleeding disorders.

  • DNA/RNA shearing service for next-gen sequencing.

  • Ultra–sensitive droplet digital PCR for detecting low–prevalence somatic mutations.

  • Absolute quantification of gene expression and alternatively spliced transcripts with digital PCR.

  • DNA and Protein Liquid Biopsy.

Equipment

  1. Full authomatised system for karyotyping and FISH (Cytovision) with 3 analysis stations.

  2. Full authomatised system for karyotyping and FISH (Metasystems) including a karyotype finder with 3 analysis stations.

  3. Microbeads-based system for cellular isolation (Miltenyi).

  4. Microscopes of light and fluorescence.

  5. Veriti 96W Thermal Cycler.

  6. Pyrosequencer (Pyromark Q24, Qiagen).

  7. Affymetrix GeneChip® Instrument System: Hybridization Oven 646/Fluidics Station 450 and GeneChip® Scanner 3000 7G.

  8. Droplet Digital PCR QX200 system (Bio–Rad Laboratories, Hercules, CA, USA).

  9. Bioanalyzer for fragment analysis: 4200 Tapestation system (Agilent technologies Santa Clara, CA).

  10. Covaris M220 Focused Ultrasonicator (Covaris, Woburn, MA, USA).

  11. Next-generation sequencing facilities (Illumina), MiSeq sequencer.